Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms
نویسندگان
چکیده
منابع مشابه
Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms
MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3'-end length variants ("isomiRs"), little attention has been paid to their differential detection by RT-qPCR. However, recent evidence indicates that 3'-end miRNA i...
متن کاملQuantification of distinct let-7 microRNA family members by a modified stem-loop RT-qPCR
Lethal-7 (let-7) microRNA (miRNA) serves a pivotal role in a number of physiological processes and is associated with the occurrence and development of multiple disorders such as cancer. The present study aimed to use a newly developed stem‑loop strategy for reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) to distinguish let‑7 miRNA family members that differ by as little ...
متن کاملmRNA and microRNA quality control for RT-qPCR analysis.
The importance of high quality sample material, i.e. non-degraded or fragmented RNA, for classical gene expression profiling is well documented. Hence, the analysis of RNA quality is a valuable tool in the preparation of methods like RT-qPCR and microarray analysis. For verification of RNA integrity, today the use of automated capillary electrophoresis is state of the art. Following the recentl...
متن کاملHuman microRNA Polyadenylation Signals
MicroRNAs (miRNAs) are small non-coding RNAs that are involved in posttranscriptional gene regulation (cf., Bartel, 2004). First identified in C. elegans just over a decade ago (Lee et al., 1993), miRNAs have been identified in virtually every metazoan and plant species examined. Experimental evidence is rapidly accumulating that shows miRNAs to play key roles in process such as cellular differ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Frontiers in Genetics
سال: 2018
ISSN: 1664-8021
DOI: 10.3389/fgene.2018.00011